Reaction vessel for carrying out array processes
AssigneeClondiag Chip Technologies GmbH
InventorsTorsten Schulz, Eugen Ermantraut, Ralf Ehricht, Klaus-Peter Möbius, Gerd Wagner, Joachim Fischer, Thomas Ellinger
Life of this patent
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Abstract
The invention relates to a reaction vessel, a device and a method for detecting specific interactions between molecular target and probe molecules. The present invention especially relates to a reaction vessel which has a shape and size typical of a laboratory reaction vessel and in which a supporting element with probe molecules immobilized thereon on predetermined regions is arranged on its base surfaces.
Claims54
A reaction vessel for detecting interactions between a molecular target and probe molecules, comprising a tube having
- a generally cylindrical free-standing wall comprising an opening configured to receive a sample and a base portion spaced apart from the opening by the cylindrical wall,
- a lid attached to the cylindrical wall via a connecting strip and configured to seal the opening and fit into an adjusting recess of a reader,
- a void located in the base portion opposed to the opening,
- a substrate secured within the void with a clamping connection or clamping sleeve to the base portion, the substrate comprising an inner surface enclosed except for the opening by the cylindrical wall and an outer surface disposed externally of the cylindrical wall, and
- probe molecules immobilised on predetermined regions of the inner surface, the probe molecules being configured to interact with the molecular target;
- wherein the connecting strip has a length,
- wherein the reaction vessel has a height of at least 1.5 cm.
A reaction vessel for detecting interactions between a molecular target and probe molecules, comprising a tube having
- a generally cylindrical free-standing wall comprising an opening configured to receive a sample and a base portion spaced apart from the opening by the cylindrical wall,
- a lid attached to the cylindrical wall via a connecting strip configured to seal the opening and fit into an adjusting recess of a reader, the lid comprising an inner surface enclosed by the tube when sealed, and
- probe molecules immobilised on predetermined regions of the inner surface having a clamping connection or clamping sleeve, the probe molecules being configured to interact with the molecular target;
- wherein the connecting strip has a length,
- wherein the reaction vessel has a height of at least 1.5 cm.
A reaction vessel comprising:
- a generally cylindrical free-standing wall comprising an opening configured to receive a sample and a base portion spaced apart from the opening by a cylindrical wall,
- a lid attached to the cylindrical wall via a connecting strip and configured to seal the opening and fit into an adjusting recess of a reader, the reaction vessel defining a volume when sealed of at least 100 μL and 2.5 mL or less,
- a void located in the base portion opposed to the opening,
- an impermeable transparent substrate secured within the void with a clamping connection or clamping sleeve to the base portion, the substrate comprising an inner surface enclosed except for the opening by the cylindrical wall and an outer surface disposed externally of the cylindrical wall, and
- probe molecules immobilized at each of multiple spaced-apart predetermined regions of the inner surface, the probe molecules of different locations being configured to interact with a different molecular target;
- wherein the connecting strip has a length,
- wherein the reaction vessel has a height of at least 1.5 cm.
Dependencies are inferred from the claim text.