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US 9,309,574 B1Granted
Molecular cloning of HIV-1 from immortalized cell lines
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsRobert C. Gallo, Flossie Wong-Staal, Mikulas Popovic, Beatrice H. Hahn, George M. Shaw, Amanda G. Fisher
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Abstract
Disclosed is the molecular cloning of HTLV-III, the adult leukemia and acquired immune deficiency syndrome (AIDS) virus. Clone BH10 contains a 9.0 Kb viral insert constituting the entire HTLV-III genome. Clones BH8 and BH5 contain viral inserts of 5.5 Kb and 3.5 Kb, respectively. These clones are suitable for the development of diagnostic and therapeutic measures for AIDS, as well as use as probes for the detection of AIDS. By scientific convention, HTLV-III, referred to herein also as HIV, has been renamed as HIV-1.
Claims161
A composition comprising a duplex formed between a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid and a single-stranded nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- wherein the duplex is outside of a mammalian cell and outside of an HIV-1 particle;
- wherein the duplex comprises a double-stranded region of at least 18 contiguous nucleotides and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein the single-stranded nucleic acid comprises a label.
12 more elements
A method for preparing a DNA construct specific for Human Immunodeficiency Virus Type-1 (HIV-1) comprising the step of inserting into a vector a nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- whereby a DNA construct comprising an inserted nucleic acid is obtained.
8 more elements
A nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid forms a duplex with an HIV-1 nucleic acid;
- and
- wherein the nucleic acid is covalently attached to a solid support.
10 more elements
A single-stranded nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with an HIV-1 nucleic acid;
- wherein the single-stranded nucleic acid is within a duplex comprising the HIV-1 nucleic acid;
- and
- wherein the duplex is covalently attached to a solid support.
11 more elements
A nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid forms a duplex with an HIV-1 nucleic acid;
- wherein the nucleic acid comprises a detectable label covalently attached to the nucleic acid;
- and
- wherein the detectable label is not an additional nucleic acid.
11 more elements
A nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid forms a duplex with an HIV-1 nucleic acid;
- wherein the nucleic acid is outside of a mammalian cell and outside of a viral particle;
- and
- wherein the nucleic acid is attached to a non-HIV-1 nucleic acid through a covalent bond.
11 more elements
A composition comprising a duplex formed between:
- (A) a single-stranded nucleic acid of between 18 and 103 contiguous nucleotides and comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- and
- (B) an HIV-1 nucleic acid selected from the group consisting of:
- (a) an HIV-1 nucleic acid comprising a nucleotide sequence encoding a full-length gag polypeptide or its complement;
- (b) an HIV-1 nucleic acid comprising a nucleotide sequence encoding a full-length pol polypeptide or its complement;
- (c) an HIV-1 nucleic acid comprising a nucleotide sequence encoding a full-length env polypeptide or its complement;
- and
- (d) an HIV-1 nucleic acid comprising a nucleotide sequence for a long terminal repeat region comprising R and U3 regions or their complements;
- wherein the single-stranded nucleic acid of (A) does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid of (A) forms a duplex with the HIV-1 nucleic acid of (B);
- wherein the duplex is outside of a mammalian cell and outside of a viral particle;
- wherein the duplex comprises a double-stranded region of between 18 and 103 contiguous nucleotides and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein the single-stranded nucleic acid comprises a label.
20 more elements
A composition comprising:
- (A) a duplex;
- and
- (B) a compound selected from the group consisting of sodium saline citrate, formamide, and dextran sulfate;
- wherein the duplex comprises a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid having a length of at least 300 nucleotides hybridized to a single-stranded nucleic acid of between 18 and 103 contiguous nucleotides and comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- and
- wherein the duplex comprises a double-stranded region and a single-stranded region that is longer than the double-stranded region.
14 more elements
A composition comprising a duplex formed between a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid and a single-stranded nucleic acid of at least 18 contiguous nucleotides and comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- wherein the single-stranded nucleic acid consists of DNA;
- wherein the duplex is outside of a mammalian cell;
- wherein the duplex comprises a double-stranded region of at least 18 contiguous nucleotides and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein the duplex is bound to a solid support.
13 more elements
A single-stranded nucleic acid of at least 18 contiguous nucleotides comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with an HIV-1 nucleic acid;
- wherein the single-stranded nucleic acid is outside of a mammalian cell and outside of a viral particle;
- wherein the single-stranded nucleic acid consists of DNA;
- and
- wherein the single-stranded nucleic acid is attached to a non-HIV-1 DNA nucleic acid through a covalent bond.
12 more elements
A composition comprising a duplex formed between:
- (A) a single-stranded nucleic acid of between 18 and 103 contiguous nucleotides and comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- and
- (B) an HIV-1 nucleic acid selected from the group consisting of:
- (a) an HIV-1 nucleic acid comprising a nucleotide sequence encoding a full-length gag polypeptide or its complement;
- (b) an HIV-1 nucleic acid comprising a nucleotide sequence encoding a full-length pol polypeptide or its complement;
- (c) an HIV-1 nucleic acid comprising a nucleotide sequence encoding a full-length env polypeptide or its complement;
- and
- (d) an HIV-1 nucleic acid comprising a nucleotide sequence for a long terminal repeat region comprising R and U3 regions or their complements;
- wherein the single-stranded nucleic acid of (A) does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid of (A) forms a duplex with the HIV-1 nucleic acid of (B);
- wherein the single-stranded nucleic acid of (A) consists of DNA;
- wherein the duplex is outside of a mammalian cell and outside of a viral particle;
- wherein the duplex comprises a double-stranded region of between 18 and 103 contiguous nucleotides and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein either the single-stranded nucleic acid or the duplex is bound to a solid support.
21 more elements
A composition comprising:
- (A) a single-stranded nucleic acid of between 18 and 103 contiguous nucleotides and comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- and
- (B) a compound selected from the group consisting of sodium saline citrate, formamide, and dextran sulfate;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with an HIV-1 nucleic acid.
11 more elements
A composition comprising a duplex formed between a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid and a single-stranded nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- wherein the duplex is outside of a mammalian cell and outside of an HIV-1 particle;
- wherein the duplex comprises a double-stranded region and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein the single-stranded nucleic acid comprises a label.
12 more elements
A composition comprising a duplex formed between a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid and a single-stranded nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- wherein the duplex is outside of a mammalian cell and outside of an HIV-1 particle;
- wherein the duplex comprises a double-stranded region and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein either the duplex or the single-stranded nucleic acid is bound to a solid support.
12 more elements
A composition comprising:
- a compound selected from the group consisting of sodium saline citrate, formamide, and dextran sulfate;
- and
- a duplex formed between a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid and a single-stranded nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- wherein the duplex is outside of a mammalian cell and outside of an HIV-1 particle;
- wherein the duplex comprises a double-stranded region and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region.
13 more elements
A composition comprising a duplex formed between a Human Immunodeficiency Virus Type-1 (HIV-1) nucleic acid and a single-stranded nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) an HIV-1 nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the single-stranded nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the single-stranded nucleic acid forms a duplex with the HIV-1 nucleic acid;
- wherein the duplex is outside of a mammalian cell and outside of an HIV-1 particle;
- wherein the duplex comprises a double-stranded region and a single-stranded region on either side of the double-stranded region that is longer than the double-stranded region;
- and
- wherein the single-stranded nucleic acid is attached to a non-HIV-1 nucleic acid through a covalent bond.
12 more elements
A nucleic acid comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid forms a duplex with an HIV-1 nucleic acid;
- and
- wherein the nucleic acid is covalently attached to a solid support.
10 more elements
A nucleic acid comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid forms a duplex with an HIV-1 nucleic acid;
- wherein the nucleic acid comprises a detectable label covalently attached to the nucleic acid;
- and
- wherein the detectable label is not an additional nucleic acid.
11 more elements
A nucleic acid comprising a nucleotide sequence selected from the group consisting of
- (i) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB2 having ATCC Accession No. 40231;
- (ii) a nucleotide sequence of either strand of viral DNA from lambda bacteriophage λ-HXB3 having ATCC Accession No. 40232;
- (iii) a nucleotide sequence of either strand of viral DNA from clone BH10 having ATCC Accession No. 40125;
- (iv) a nucleotide sequence of either strand of viral DNA from clone BH5 having ATCC Accession No. 40126;
- (v) a nucleotide sequence of either strand of viral DNA from clone BH8 having ATCC Accession No. 40127;
- (vi) a nucleotide sequence of either strand of viral DNA from clone pHXB3 having ATCC Accession No. 67081;
- (vii) a nucleotide sequence of either strand of viral DNA from clone pHXB-2D having ATCC Accession No. 67082;
- (viii) a nucleotide sequence of either strand of viral DNA from E. coli clone X10-1 having ATCC Accession No. 67083;
- and
- (ix) a Human Immunodeficiency Virus Type-1 (HIV-1) nucleotide sequence from the H9/HTLV-III cell line having ATCC Accession No. CRL 8543;
- wherein the nucleic acid does not form a duplex with HTLV-I and HTLV-II nucleic acids under conditions of stringency for hybridization under which the nucleic acid forms a duplex with an HIV-1 nucleic acid;
- wherein the nucleic acid is outside of a mammalian cell and outside of a viral particle;
- and
- wherein the nucleic acid is attached to a non-HIV-1 nucleic acid through a covalent bond.
11 more elements
Dependencies are inferred from the claim text.