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US 9,309,542 B2Granted
Recombinant Caldicellulosiruptor bescii and methods of use
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsMinseok Cha, Janet Westpheling, Daehwan Chung
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Abstract
This disclosure describes recombinant Caldicellulosiruptor bescii microbes designed to produce greater amounts of acetate, H2, and/or ethanol than a comparable wild type control. this disclosure also describes methods that generally include growing such recombinant microbes under conditions effective for the recombinant microbes to produce acetate, H2, and/or ethanol.
Claims11
A recombinant Caldicellulosiruptor microbe genetically modified to produce a greater amount of ethanol than a comparable wild type control, wherein the Caldicellulosiruptor microbe:
- comprises a knock-out of the coding region of a lactate dehydrogenase and produces lactate in an amount less than a comparable wild type control;
- comprises at least one heterologous polynucleotide that encodes an enzyme in a metabolic pathway that converts acetyl-CoA to ethanol, wherein the enzyme is an acetaldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, an aldehyde dehydrogenase that can convert acetyl-CoA to acetaldehyde, or a combination thereof;
- and wherein the Caldicellulosiruptor microbe is C. bescii, C. kristjansonii, C. obsidiansis, C. hydrothermalis, C. kronotskvensis, C. lactoaceticus, or C. saccharolvticus.
Dependencies are inferred from the claim text.