Beta-glucosidase variants having improved activity, and uses thereof
AssigneesIfp Energies Nouvelles, Proteus S.A.
InventorsNicolas Lopes-Ferreira, Antoine Margeot, Hugues Mathis, Laurent Fourage
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Abstract
The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more particularly to beta-glucosidase variants comprising at least one modification among the amino acids located at positions 225, 238, 240 and 241, according to the numbering in SEQ ID No. 2 of Trichoderma reesei beta-glucosidase, and also relates to the use of said variants having improved effectiveness in methods for cellulose decomposition and biofuel production.
Claims11
An isolated or purified modified polypeptide comprising an amino acid sequence in which at least one amino acid is modified compared with the amino acid sequence SEQ ID No. 2, said modified amino acid being chosen from positions 225, 238, 240 and 241 of the amino acid sequence SEQ ID No. 2, wherein said modified polypeptide has the amino acid sequence SEQ ID No. 10;
- or at least 85% identity with said amino acid sequences SEQ ID No. 2 or SEQ ID NO. 10, and wherein said modified polypeptide has an improved beta-glucosidase activity compared with the beta-glucosidase activity of the wild-type protein having the sequence set forth in SEQ ID No. 2.
Dependencies are inferred from the claim text.