Method for preparing an industrial yeast, industrial yeast, and application to the production of ethanol from at least one pentose
AssigneeLesaffre Et Compagnie
InventorsThomas Desfougeres, Georges Pignede
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Abstract
The present invention relates to methods for obtaining ethanol-producing yeast strains, yeast strains thus produced, and the industrial production of ethanol from said strains. More particularly, the present invention describes in its most general aspect, a method for preparing yeasts from Saccharomyces cerevisiae strains by means of the integration, into the genome of the yeast, of at least one gene encoding xylose isomerase and of at least one gene encoding xylitol dehydrogenase. The strains of the invention are useful for producing ethanol from a medium comprising at least one pentose, preferably xylose or a mixture of xylose and arabinose.
Claims30
A yeast strain comprising exogenous enzyme genes of the xylose metabolic pathway consisting of at least one copy of an exogenous gene encoding a xylose isomerase, and at least one copy of an exogenous gene encoding a xylitol dehydrogenase, wherein the exogenous gene encoding a xylose isomerase and the exogenous gene encoding a xylitol dehydrogenase are the only exogenous enzyme genes of the xylose metabolic pathway present in the yeast strain, and wherein the exogenous gene encoding a xylose isomerase is a gene from Clostridium, Piromyces, Bacteroides, Streptomyces, Haemophilus, Burkholderia, Enterococcus, Thermotoga, Fusobacterium, Geobacillus, Arthrobacter, Ciona, Physcomitrella, Cellvibrio, Chitinophaga, Saccharopolyspora or Salinibacter.
A yeast strain, wherein said strain is obtained by introducing in a starting yeast strain at least one copy of an exogenous gene encoding a xylose isomerase, and at least one copy of an exogenous gene encoding xylitol dehydrogenase, wherein the exogenous gene encoding a xylose isomerase and the exogenous gene encoding a xylitol dehydrogenase are the only exogenous enzymes genes of the xylose metabolic pathway to be introduced in the starting yeast strain, and wherein the exogenous gene encoding a xylose isomerase is a gene from Clostridium, Piromyces, Bacteroides, Streytomyces, Haemophilus, Burkholderia, Enterococcus, Thermotoga, Fusobacterium, Geobacillm, Arthrobacter, Ciona, Physcomitrella, Cellvibrio, Chitinoyham, Saccharovolysvora or Salinibacter.
Dependencies are inferred from the claim text.