Nannochloropsis kozak consensus sequence
AssigneeExxonMobil Research and Engineering Company
InventorsPeter DeHoff, Leah Soriaga
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Abstract
The present invention provides Nannochloropsis consensus Kozak sequences for use in protein expression in eukaryotic cells, such as algal cells. The invention further provides expression constructs, expression cassettes, cloning or expression vectors and host eukaryotic cells, such as algal cells, and methods for expressing proteins of interest which take advantage of the consensus Kozak sequences as described herein.
Claims23
A recombinant DNA molecule comprising a Kozak sequence selected from the group consisting of SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, and SEQ ID NO:17, operably linked to a heterologous nucleotide sequence encoding a polypeptide, wherein positions 1, 2, and 3 of the Kozak sequence comprise the initiating methionine codon of the polypeptide, and wherein the heterologous nucleotide sequence encoding a polypeptide is codon optimized for expression in an algal or heterokont species.
Dependencies are inferred from the claim text.