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US 9,309,512 B2Granted
Phased small RNAs
AssigneeMonsanto Technology LLC
InventorsEdwards Allen, Liang Guo, Sara E. Heisel, Sergey I. Ivashuta, Yuanji Zhang
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Abstract
This invention discloses recombinant DNA constructs encoding phased small RNAs useful in regulating expression of one or more genes of interest. Also disclosed by this invention are transgenic plant cells, plants, and seeds containing a recombinant DNA construct of this invention.
Claims2
A recombinant DNA construct comprising a promoter operably linked to DNA that transcribes to RNA comprising:
- (a) at least one exogenous recognition site recognizable by a phased small RNA expressed in a specific plant cell of a plant, and
- (b) target RNA to be suppressed in said specific plant cell,
- wherein said target RNA is to be expressed in cells of said plant other than said specific plant cell,
- wherein said phased small RNA has a nucleotide sequence that maps to the plus strand of an endogenous phased small RNA locus,
- wherein said phased small RNA locus contains at least three contiguous phased small RNAs,
- wherein said at least three contiguous phased small RNAs target multiple genes or different segments of a single gene, and wherein said phased small RNA locus is cleaved in phase by a plant DCL4 ribonuclease.
3 more elements
Dependencies are inferred from the claim text.