Methods and compositions for sweet corn sugary enhancer (SEI) gene
AssigneeSyngenta Participations AG
InventorsMercedes Murua, David J Plunkett, Robert Dietrich, Molly Dunn, Gayle Dace
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Abstract
The present invention relates to methods and compositions for identifying, selecting and/or producing a corn plant or corn germplasm comprising a corn sugary enhancer gene (SE1) with enhanced sugary trait. A corn plant, part thereof and/or corn germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided.
Claims11
A method of identifying a corn plant or corn germplasm comprising an enhanced sugary gene locus, said method comprising:
- a) detecting in an amplification reaction, in said plant or germplasm genomic DNA, a deletion located on maize chromosome 2 comprising SEQ ID NO:
- 4;
- and
- b) identifying a corn plant or corn germplasm comprising the enhanced sugary gene locus.
A method of producing a corn plant that produces kernels with enhanced sugary trait, said method comprising:
- a) detecting in an amplification reaction, in corn germplasm genomic DNA that is homozygous for the sugary (su1) mutant allele, the presence of a homozygous deletion located on maize chromosome 2 comprising SEQ ID NO:
- 4;
- and
- b) producing a plant from said corn germplasm, thereby producing a corn plant with enhanced sugary trait.
A method of selecting a corn plant or corn germplasm comprising an enhanced sugary gene locus, said method comprising:
- a) crossing a first corn plant or corn germplasm with a second corn plant or corn germplasm to produce progeny, wherein the first corn plant or corn germplasm comprises a deletion located on maize chromosome 2 comprising SEQ ID NO:
- 4,
- b) detecting in an amplification reaction, in genomic DNA from one or more of said progeny, the presence of said deletion;
- and
- c) selecting from said progeny of b) a corn plant or corn germplasm that comprises said deletion, thereby selecting a corn plant or corn germplasm comprising the enhanced sugary gene locus.
A method of introgressing an enhanced sugary gene locus into a corn plant having a genetic background lacking said gene locus, said method comprising:
- a) crossing a donor comprising said gene locus with a recurrent parent that lacks said gene locus to produce progeny;
- and
- b) repeatedly backcrossing progeny comprising said gene locus with the recurrent parent, wherein said progeny are identified by detecting the presence in an amplification reaction, in their genomic DNA, of a deletion comprising SEQ ID NO:
- 4 located on maize chromosome 2, thereby producing a corn plant comprising an enhanced sugary gene locus, and thereby introgressing said gene locus into a corn plant having a genetic background lacking said gene locus.
Dependencies are inferred from the claim text.