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US 9,309,505 B2Granted
Polypeptides having glucoamylase activity and polynucleotides encoding same
AssigneesNovozymes North America, Inc., Novozymes A/S
InventorsSara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
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Abstract
The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims21
A process of producing a fermentation product from starch-containing material comprising the steps of:
- (a) liquefying starch-containing material;
- (b) saccharifying the liquefied material;
- and
- (c) fermenting with a fermenting organism;
- wherein step (b) is carried out using at least a
- polypeptide having glucoamylase activity selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO:
- 2.
8 more elements
A process of producing a fermentation product from starch-containing material, comprising the steps of:
- (a) saccharifying starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material;
- and
- (b) fermenting with a fermenting organism,
- wherein step (a) is carried out using at least
- a polypeptide having glucoamylase activity selected from the group consisting of:
- (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the catalytic domain shown as amino acids 21 to 472 of SEQ ID NO:
- 2.
7 more elements
A method of producing a polypeptide comprising:
- (a) cultivating a host cell comprising a heterologous nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide;
- and (b) recovering the polypeptide, wherein the polypeptide has glucoamylase activity and comprises an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.