Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsLene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Abstract
The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
Claims24
A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase I activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide has at least 80% sequence identity with amino acids 1 to 532 of SEQ ID NO:
- 56.
A method of producing a polypeptide having cellobiohydrolase I activity, said method comprising:
- (a) cultivating a recombinant host cell comprising a polynucleotide encoding a polypeptide having cellobiohydrolase I activity under conditions conducive for production of the polypeptide;
- and (b) recovering the polypeptide;
- wherein the polypeptide has at least 80% sequence identity with amino acids 1 to 532 of SEQ ID NO:
- 56.
A method for producing ethanol from biomass, said method comprising:
- (a) contacting the biomass with the polypeptide having cellobiohydrolase I activity, wherein the polypeptide has at least 80% sequence identity with 1 to 532 of SEQ ID NO:
- 56;
- (b) converting the degraded biomass to ethanol;
- and (c) recovering the ethanol.
A recombinant host cell transformed with a nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having cellobiohydrolase I activity, wherein the polypeptide having cellobiohydrolase I activity has at least 80% sequence identity with amino acids 1 to 532 of SEQ ID NO:
- 56.
Dependencies are inferred from the claim text.