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AssigneeDPx Holdings B.V.
InventorsMartin Kietzmann, Helmut Schwab, Harald Pichler, Mirela Ivancic, Oliver May, Rudolf Gijsbertus Marie Luiten
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The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.
A method of producing a protein with esterase activity comprising expressing a gene encoding the protein in an E. coli strain, wherein the gene encoding the protein with esterase activity has at least 99% identity to the polynucleotide of SEQ ID NO 11 and encodes a protein that has at least 99% identity to SEQ ID NO 12 and wherein the expression takes place without coexpression of GroEL and/or GroES from a plasmid.
A method of producing a protein with esterase activity comprising expressing a gene encoding the protein in an E. coli strain, wherein the gene encoding the protein with esterase activity has at least 99% identity to the polynucleotide of SEQ ID NO 11 and encodes a protein that has at least 99% identity to SEQ ID NO 12.
Dependencies are inferred from the claim text.