Method for expansion of stem cells and the use of such cells
AssigneeThe Research Foundation for The State University of New York
InventorYupo Ma
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Abstract
The present invention demonstrates that SALL4A and SALL4B are strong positive regulators of hematopoetic stem cell expansion. HSCs receiving expression of SALL4A or SALL4B are able to achieve a high-level of expansion. Cultures of SALL4-transduced cells results in extensive HSC expansion with over 1000-fold higher levels than controls within 2 to 3 weeks and expanded HSCs show no or very little maturation. Moreover, the expansion occurs quite rapidly with significant HSC growth in just a few days. In addition, SALL4-induced HSC expansion exhibits no impairment of hematopoietic cell differentiation. SALL4 appears to function in the maintenance of an undifferentiated proliferation state and block cell differentiation for HSCs.
Claims15
A method for expanding a hematopoietic stem cell population, the method comprising providing to the stem cell population a Sal-like 4 (SALL4) polypeptide attached to a transport moiety capable of crossing a cell membrane, in an amount effective to expand the stem cell population.
A method for expanding a hematopoietic stem cell population ex vivo, the method comprising providing to the ex vivo stem cell population Sal-like 4 (SALL4) polypeptide in an amount effective to expand the stem cell population.
A method for treatment of a disorder in a subject requiring a hematopoietic stem cell or an expanded hematopoietic stem cell, the method comprising:
- a) obtaining a hematopoietic stem cell population,
- b) providing to the stem cell population a composition comprising a Sal-like 4 (SALL4) polypeptide in an effective amount for the expansion of the stem cell population, and
- c) transplanting the expanded stem cell population to the subject in an amount effective for the treatment of said disorder.
Dependencies are inferred from the claim text.