Cell culture apparatus for co-culture of cells

A multi-layer cell co-culture culture vessel comprising:

• a first group of a plurality of cell culture chambers in a multi-layer cell culture vessel, wherein each of the plurality of cell chambers of the first group has an aperture;
• a second group of a plurality of cell culture chambers in a multi-layer cell culture vessel;
• wherein each of the plurality of cell chambers of the second group has an aperture;
• at least one tracheal chamber adjacent to two cell culture chambers and separated from the adjacent cell culture chambers by a gas permeable, liquid impermeable membrane;
• a manifold comprising two apertures, a first aperture and a second aperture, and a divider which separates a first port from a second port,
• wherein the first aperture provides access to the first group of cell culture chambers through the ports, but does not provide access to the second group of cell culture chambers, and
• wherein the second aperture provides access to the second group of cell culture chambers through the apertures, but does not provide access to the second group of cell culture chambers.