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US 9,309,314 B2Granted
Polypeptides, nucleic acids and uses thereof
AssigneeAgency for Science, Technology and Research (A*Star)
InventorBruno Reversade
Life of this patent
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Abstract
We describe an ELABELA polypeptide comprising a sequence CXXXRCXXXHSRVPFP (SEQ ID NO: 1), in which X signifies an amino acid residue, such as a sequence selected from the group consisting of: SEQ ID NO: 2 to SEQ ID NO: 18, preferably CLQRRCMPLHSRVPFP (SEQ ID NO: 2), or a fragment, homolog, variant or derivative thereof, which polypeptide is capable of maintaining self-renewal and/or pluripotency of a stem cell.
Claims23
An ELABELA polypeptide fragment comprising a sequence CXXXRCXXXHSRVPFP (SEQ ID NO:
- 1), wherein X is an/any amino acid residue, said polypeptide fragment further comprising a label, wherein an intramolecular covalent bond is present between the cysteine residues at positions 1 and 6 of SEQ ID NO:
- 1, and wherein the polypeptide fragment maintains self-renewal, pluripotency, or both of a stem cell.
A method of making an ELABELA polypeptide or fragment thereof, the method comprising:
- (a) expressing a nucleic acid encoding a sequence comprising CXXXRCXXXHSRVPFP (SEQ ID NO:
- 1) from a nucleic acid vector in a cell, wherein X is an/any amino acid residue, and wherein the polypeptide fragment maintains self-renewal, pluripotency, or both of a stem cell;
- or
- (b) using chemical synthesis to generate a synthetic polypeptide or fragment thereof comprising CXXXRCXXXHSRVPFP (SEQ ID NO:
- 1) or a fragment having the sequence of SEQ ID NO:
- 53, wherein X is an/any amino acid residue, and wherein the polypeptide fragment maintains self-renewal, pluripotency, or both of a stem cell.
3 more elements
Dependencies are inferred from the claim text.