Water soluble membrane proteins and methods for the preparation and use thereof
AssigneeMassachusetts Institute of Technology
InventorsShuguang Zhang, Alexander Rich, Karolina Corin, Lotta T. Tegler
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Abstract
The present invention is directed to water-soluble membrane proteins, methods for the preparation thereof and methods of use thereof.
Claims13
A water-soluble variant of a G-protein coupled receptor (GPCR), wherein in said variant, 7-transmembrane α-helical hydrophobic residues Leucine (L), isoleucine (I), valine (V), and phenylalanine (F) in hydrophilic surface α-helical positions b, c, and f but not positions a, d, e, and g of the GPCR have been substituted by glutamine (Q), threonine (T), threonine (T), and tyrosine (Y), respectively, optionally further comprising conservative substitutions at other parts of the variant.
A water soluble variant of a G-protein coupled receptor (GPCR), wherein at least one transmembrane domain has been substituted with a peptide selected from the group consisting of SEQ ID NOs. 3, 4, 5, 6, 7, or 8.
Dependencies are inferred from the claim text.