Chromosomal insertion of GFP into bacteria for quality control
AssigneesMacquarie University, BTF Pty Ltd
InventorsLeonardo B. Pinheiro, Peter Bergquist, Moreland Gibbs, Graham Vesey
Life of this patent
Estimating the term…
Abstract
An isolated mutated green fluorescent protein (gfp) gene for chromosomal insertion into a bacterium, wherein the gene is capable of being expressed in bacteria and produce sufficient fluorescence under illumination from a UV lamp in a bacterial colony to be seen by the naked eye. A gene cassette for inserting a gene into a chromosome.
Claims13
An isolated modified bacterium forming a colony having fluorescence visible by the naked eye when the colony is illuminated by ultraviolet light, the modified bacterium comprising a mutated green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria inserted into the chromosome of the modified bacterium;
- wherein the mutated GFP gene comprising a nucleic acid molecule consisting of mutations at nucleotide positions 1737 and 1739 of SEQ ID NO:
- 4 encoding Ser72Ala, nucleotide position 1981 of SEQ ID NO:
- 4 encoding Met153Thr and nucleotide position 2011of SEQ ID NO:
- 4 encoding Val163Ala, or the mutated GFP gene comprising a nucleic acid molecule consisting of mutations which encode amino acid changes Ser72Ala, Met153Thr, Val163 Ala of SEQ ID NO:
- 3, and wherein the mutated GFP gene is under the control of an un-repressed strong promoter.
An isolated modified bacterium forming a colony having fluorescence visible by the naked eye when the colony is illuminated by ultraviolet light, the modified bacterium comprising a mutated green fluorescent protein (GPP) gene from the jellyfish Aequorea victoria inserted into the chromosome of the modified bacterium, the modified bacterium is selected from the group consisting of National Measurement Institute (formerly Australian Government Analytical Laboratories) deposit accession numbers NM04/42817, NM04/42818, NM04/42819, and NM04/42820.
Dependencies are inferred from the claim text.