DNA sequence, and recombinant preparation of group 4 major allergens from cereals
AssigneeMerck Patent GmbH
InventorsAndreas Nandy, Helmut Fiebig, Oliver Cromwell
Life of this patent
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Abstract
The present invention relates to the provision of DNA sequences of group 4 major allergens from cereals. The invention also encompasses fragments, new combinations of partial sequences and point mutants having a hypoallergenic action. The recombinant DNA molecules and the derived polypeptides, fragments, new combinations of partial sequences and variants can be utilized for the therapy of pollen-allergic diseases. The proteins prepared by recombinant methods can be employed for in vitro and in vivo diagnosis of pollen allergies.
Claims11
An isolated DNA molecule comprising a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8 and SEQ ID NO:
- 10, wherein said nucleic acid sequence has been codon-optimized for expression of the polypeptide in a host cell that is not a cell of Secale cereale Hordeum vulgare or Triticum aestivum.
A recombinant DNA expression vector or a cloning system comprising an isolated DNA molecule selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7 and SEQ ID NO:
- 9, and a heterologous expression control sequence.
Dependencies are inferred from the claim text.