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US 9,309,295 B1Granted
Genetically modified filamentous fungi and uses thereof
AssigneeDanmarks Tekniske Universitet
InventorsLars Poulsen, Jette Thykær, Anna Eliasson Lantz
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Abstract
The present invention relates to a filamentous fungus comprising a genetic modification in one more hydrophobin genes and the use of the same as host cell for preparing a biosynthetic product. The present invention further pertains to a vector for and methods for generating said filamentous fungus.
Claims10
A filamentous fungus comprising a genetic modification in at least one hydrophobin gene,
- wherein said at least one hydrophobin gene encodes a polypeptide comprising an amino acid sequence selected from the group consisting of:
- the amino acid sequence having the sequence set forth in SEQ ID #1, an amino acid sequence having at least 85% sequence identity to SEQ ID #1, the amino acid sequence having the sequence set forth in SEQ ID #7, an amino acid sequence having at least 85% sequence identity to SEQ ID #7, the amino acid sequence set forth in SEQ ID #4 and an amino acid sequence having at least 85% sequence identity to SEQ ID #4,
- wherein said genetic modification reduces or abolishes the activity or expression of said hydrophobin,
- wherein said fungus is an Aspergillus spp and with the proviso that not all hydrophobin genes of said fungus are genetically modified,
- wherein said fungus further comprises at least one transgene, and
- wherein said transgene is encoded by an expression vector adapted for expression of said transgene.
3 more elements
Dependencies are inferred from the claim text.