Computationally optimized broadly reactive antigens for H1N1 influenza
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsTed M. Ross, Corey J. Crevar, Donald M. Carter, Jr.
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Abstract
The generation of optimized H1N1 influenza HA polypeptides for eliciting a broadly reactive immune response to H1N1 influenza virus isolates is described. The optimized HA polypeptides were developed through a series of HA protein alignments, and subsequent generation of consensus sequences, based on selected H1N1 viruses isolated from 1918-2012. Optimized H1N1 HA polypeptides, and compositions, fusion proteins and VLPs comprising the HA polypeptides are described. Condon-optimized nucleic acid sequences encoding the HA polypeptides are also described. Methods of eliciting an immune response against influenza virus in a subject are also described.
Claims16
A recombinant influenza hemagglutinin (HA) polypeptide, comprising:
- (i) the amino acid sequence of SEQ ID NO:
- 1 or the amino acid sequence of residues 2-566 of SEQ ID NO:
- 1;
- (ii) the amino acid sequence of SEQ ID NO:
- 2 with no more than 2 amino acid substitutions relative to SEQ ID NO:
- 2 or the amino acid sequence of residues 2-566 of SEQ ID NO:
- 2;
- (iii) the amino acid sequence of SEQ ID NO:
- 3 or the amino acid sequence of residues 2-566 of SEQ ID NO:
- 3;
- (iv) the amino acid sequence of SEQ ID NO:
- 5 or the amino acid sequence of residues 2-566 of SEQ ID NO:
- 5;
- (v) the amino acid sequence of SEQ ID NO:
- 6 with no more than 4 amino acid substitutions relative to SEQ ID NO:
- 6 or the amino acid sequence of residues 2-565 of SEQ ID NO:
- 6;
- or
- (vi) the amino acid sequence of SEQ ID NO:
- 7 or the amino acid sequence of residues 2-566 of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.