Borate moiety-contained linker and bio-sensing element containing the same

A borate moiety-contained linker for modifying a sensing molecule and connecting the sensing molecule to a substrate, the linker comprising a compound having a structure represented by the following chemical formula (I):

• wherein
• R1 is one of the following chemical structures:
• X is selected from the group consisting of ester bond (—COO— and —OCO—), 1,3-sucinimide bond
• and triazole bond
• and
• L is ester bond (—COO— and —OCO—), and
• Z is selected from the group consisting of amide bond (—CONH— and —NHCO—) ester bond (—COO— and —OCO—), 1,3-succinimide bond
• and triazole bond
• and X, Z and L are different to each other,
• when X is the 1,3-succinimide bond or the triazoie bond, N atom of the 1,3-succinimide bond or the triazole bond is attached to the aryl ring, and
• when Z is the 1,3-succinimide bond, N atom of the 1,3-succinimide bond is attached to the alkyl group connected to X,
• Y is thiol group (—SH), amine group (—NH2), azido group (—N3), carboxylic acid group (—COOH) or disulfide-contained group (—S—S—R2),
• R2 is pyridine
• or 1,2-dithiolane-contained group
• R3 is methylene group (—CH2—) or N—(3H-1,2,3,-triazo)4-methyl pentanamide
• R4 is hydrogen (H) or nitro group (—NO2),
• m is an integral from 1 to 10,
• n is an integral from 0 to 10, and
• o is 1 or 2.