Sequential delivery device and method

A reagent delivery device comprising:

• at least one reagent delivery column having a housing, the housing including a housing volume;
• at least one reagent storage element configured to store reagent and movably positioned in the housing volume;
• a breaching element coupled to the housing and configured to breach the at least one reagent storage element, at least one of the breaching element and the housing configured to communicate reagent from at least one of the housing volume and the reagent storage element, to an external volume located externally with respect to the housing volume;
• a centrifugal device configured to rotate, the reagent delivery column selectively positionable in the centrifugal device, rotation of the centrifugal device producing centrifugal forces moving the at least one reagent element toward the breaching element when in operation;
• and
• a target chamber coupled to the reagent delivery column and forming the external volume in fluid communication with at least one of the breaching element and the housing volume, the target chamber having an active element column, a receptacle, and a collection volume, the active element column configured to be selectively in fluid communication between the receptacle and collection volume, respectively.