Methods and compositions for delivering interleukin-1 receptor antagonist
AssigneeBiomet Biologics, LLC
InventorsJoel C. Higgins, Jennifer E. Woodell-May, Jacy C. Hoeppner
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Abstract
Methods and compositions generating and using an interleukin-1 receptor antagonist (IL-1ra)-rich solution. Methods for generating and isolating interleukin-1 receptor antagonist include incubating adipose tissue and/or adipocytes with polyacrylamide beads to produce interleukin-1 receptor antagonist. The interleukin-1 receptor antagonist is isolated from the polyacrylamide beads to obtain the solution rich in interleukin-1 receptor antagonist. Methods for treating a site of inflammation in a patient include administering to the site of inflammation the solution rich in interleukin-1 receptor antagonist.
Claims23
A method of treating a site of inflammation in a patient comprising:
- (a) contacting a liquid volume comprising isolated adipose tissue with polyacrylamide beads, wherein the polyacrylamide beads activate the isolated adipose tissue to produce interleukin-1 receptor antagonist to form a solution rich in interleukin-1 receptor antagonist;
- (b) separating the solution rich in interleukin-1 receptor antagonist from the isolated adipocytes and the polyacrylamide beads;
- and
- (c) administering the solution rich in interleukin-1 receptor antagonist to the site of inflammation in the patient.
A method of treating an inflammatory disorder in a patient, the method comprising:
- (a) obtaining adipose tissue from the patient;
- (b) loading the adipose tissue into a concentrator assembly including polyacrylamide beads and incubating the mixture of beads and adipose tissue to form a solution rich in interleukin-1 receptor antagonist;
- (c) rotating the concentrator assembly at centrifugal speeds to separate the polyacrylamide beads from the solution rich in interleukin-1 receptor antagonist;
- (d) obtaining whole blood from the patient;
- (e) loading the whole blood and a calcium solution into a blood isolation device;
- (f) heating the whole blood for at least about 20 minutes, at a temperature of at least about 20° C.;
- (g) centrifuging the heated whole blood and obtaining a clotting fraction;
- and
- (h) administering the solution rich in interleukin-1 receptor antagonist and the clotting fraction to the site of the inflammation in the patient.
A method of treating a site of inflammation in a patient comprising:
- (a) obtaining a liquid volume comprising adipocytes and a liquid volume comprising white blood cells from the patient;
- (b) contacting the liquid volume comprising adipocytes and a liquid volume comprising white blood cells with polyacrylamide beads;
- (c) separating the liquid volume from the polyacrylamide beads, the adipocytes and the white blood cells to obtain a solution rich in interleukin-1 receptor antagonist;
- and
- (d) administering the solution rich in interleukin-1 receptor antagonist to the site of inflammation in the patient.
Dependencies are inferred from the claim text.