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US 9,307,776 B2Granted
Pregastric esterase and derivatives thereof
AssigneeDsm IP Assets B.V.
InventorsLucie Parenicova, Ronald Busink, Jan Metske Van Der Laan
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Abstract
The present invention relates to novel lipase polynucleotide sequences, their corresponding proteins as well as ways of manufacturing said sequences and said proteins and use of the proteins in the preparation of food compositions. The invention further relates to methods for releasing proteins from the exterior of a host cell as well as to a method for killing micro-organisms.
Claims13
A recombinant polypeptide having lipolytic activity comprising:
- an amino acid sequence, which is at least 80% homologous to the amino acid sequence of SEQ ID NO:
- 2, wherein the amino acid sequence of the recombinant polypeptide comprises, when compared to SEQ ID NO:
- 2, amino acid substitutions of A70S, K98N, R158N, R159K, H318N, P320S, and I361T.
Dependencies are inferred from the claim text.