US 9,307,707 B2 · Soy gene cluster regions and methods of use

Abstract

Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to pathogens; soybean plants, seeds, and tissue cultures produced by any of the disclosed methods; seed produced by the disclosed soybean plants; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.

Claims7

Dependent claims

A method of identifying a Glycine max soybean plant (‘soybean plant’) that displays resistance to a Glycine max aphid (‘soybean aphid’), the method comprising:

a. detecting by nucleic acid assay in the soybean plant at least one allele of a marker locus associated with the resistance to a soybean aphid wherein the marker locus comprises SEQ ID NO:1, wherein SEQ ID NO:1 comprises an A at nucleotide position 249;
and
b. identifying the soybean plant or selecting a progeny of the soybean plant whereby the resulting plant displays resistance to a soybean aphid, wherein said soybean plant or progeny comprises SEQ ID NO:1 having an A at nucleotide position 249.

Dependencies are inferred from the claim text.

Facts

Application

13/687,593Filed 28 Nov 2012 · us provisional application

Priority

16 Mar 2012

Published

US 2014/0026251 A123 Jan 2014

Granted

12 Apr 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 5/10
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: seeds
A01H 6/542
Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut: glycine max [soybean]
C12N 15/8279
For stress resistance, e.g. heavy metal resistance: for biotic stress resistance, pathogen resistance, disease resistance

Soy gene cluster regions and methods of use

Life of this patent

Soy gene cluster regions and methods of use

Life of this patent

Abstract

Claims7