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US 9,304,084 B2Granted
Spatial positioning of spectrally labeled beads
AssigneeLife Technologies Corporation
InventorsStephen Empedocles, Andrew Watson, Jian Jin
Life of this patent
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Abstract
Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
Claims20
A multiplex assay, comprising:
- an assay substrate;
- and
- a plurality of spectrally labeled bodies affixed to the assay substrate in a spatially resolved configuration, wherein each of the spectrally labeled bodies includes a plurality of different markers used to encode discrete and different emission spectra, wherein each emission spectrum has a plurality of signals at differing wavelengths.
A multiplex assay apparatus, comprising:
- a sample container holding a sample fluid containing a plurality of spectrally labeled bodies;
- and
- a fluidics assembly configured to wash away spectrally labeled bodies not bound to an identifiable substance, transfer the bound spectrally labeled bodies to a reading container and affix the bound spectrally labeled bodies to the reading container in a spatially resolved configuration, wherein each of the bound spectrally labeled bodies includes a plurality of different markers used to encode discrete and different emission spectra, wherein each emission spectrum has a plurality of signals at differing wavelengths.
A method for making a multiplex assay, comprising:
- affixing a plurality of spectrally labeled bodies to an assay substrate in a spatially resolved configuration, wherein each of the spectrally labeled bodies include a plurality of different markers used to encode discrete and different emission spectra, wherein each emission spectrum has a plurality of signals at differing wavelengths.
Dependencies are inferred from the claim text.