Compositions and methods for RNA hybridization applications
AssigneeDako Denmark A/S
InventorSteen Hauge Matthiesen
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Abstract
The invention provides methods and compositions for hybridizing at least one molecule to an RNA target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in RNA hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to enable hybridization to RNA.
Claims54
A composition comprising at least one polar aprotic solvent in an amount effective to enable hybridization of a nucleic acid sequence to an RNA sequence within a cell in a sample having a preserved cell morphology, and at least 10% dextran sulfate, wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO).
A hybridization method comprising:
- combining a sample having a preserved cell morphology comprising an RNA sequence, a nucleic acid sequence, and an aqueous composition for at least a time period sufficient to hybridize the RNA sequence within the cell with the nucleic acid sequence,
- wherein the aqueous composition comprises at least one polar aprotic solvent in an amount effective to enable hybridization of the nucleic acid sequence to an RNA sequence within a cell and the sample morphology is preserved, and at least 10% dextran sulfate, wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO).
Dependencies are inferred from the claim text.