Recombinant baculovirus and use thereof
AssigneeSysmex Corporation
InventorsTakahiko Bando, Mutsumi Sugai
Life of this patent
Estimating the term…
Abstract
The present invention provides a recombinant baculovirus. The baculovirus has a genome into which a gene encoding γ-glutamyl carboxylase (GGCX) and a gene encoding DT-diaphorase (NQO1) are incorporated. The present invention further provides a method for producing a recombinant vitamin K-dependent protein by using the recombinant baculovirus.
Claims17
A recombinant baculovirus, wherein a gene encoding γ-glutamyl carboxylase (GGCX) and a gene encoding DT-diaphorase (NQO1) are incorporated into a genome of the recombinant baculovirus.
A kit for producing a recombinant vitamin K-dependent protein, comprising a recombinant baculovirus, wherein a gene encoding GGCX and a gene encoding NQO1 are incorporated into a genome of the recombinant baculovirus.
A method for producing a recombinant vitamin K-dependent protein, comprising expressing a γ-glutamyl carboxylated vitamin K-dependent protein in a lepidopteran insect or cultured cell of the lepidopteran insect that expresses a γ-glutamyl carboxylated vitamin K-dependent protein and has been infected with a recombinant baculovirus, wherein a gene encoding GGCX and a gene encoding NQO1 are incorporated into a genome of the recombinant baculovirus.
Dependencies are inferred from the claim text.