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US 9,297,028 B2Granted
Fermentive production of four carbon alcohols
AssigneeButamax Advanced Biofuels LLC
InventorsGail K. Donaldson, Lixuan Lisa Huang, Lori Ann Maggio-Hall, Vasantha Nagarajan, Charles E. Nakamura, Wonchul Suh
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Abstract
Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.
Claims54
A recombinant microbial host cell comprising heterologous DNA molecules encoding polypeptides that catalyze substrate to product conversions for each step below:
- a) acetyl-CoA to acetoacetyl-CoA;
- b) acetoacetyl-CoA to 3-hydroxybutyryl-CoA;
- c) 3-hydroxybutyryl-CoA to crotonyl-CoA;
- d) crotonyl-CoA to butyryl-CoA;
- and
- e) butyryl-CoA to butyraldehyde,
- wherein said microbial host cell produces 1-butanol by the action of an endogenous alcohol dehydrogenase, wherein said microbial host cell produces 1-butanol through the substrate to product conversions of (a) to (e) under aerobic conditions.
4 more elements
Dependencies are inferred from the claim text.