# US 9,290,820 B2 · Methods for detection of parvovirus B19

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- Number: US 9,290,820 B2
- Document ID: US9290820B2
- Status: Granted
- Kind: B2
- Application number: 14/053,245
- Filed: 2013-10-14
- Published: 2014-04-17
- Granted: 2016-03-22
- Earliest claimed: 2007-01-16
- Assignees: Grifols Therapeutics Inc.
- Applicants: Grifols Therapeutics Inc.
- Inventors: Douglas C. Lee; Todd M. Gierman; Chris Glenn; Burton Beams; Brett Buno; Lori Rinckel
- Web page: https://patentgrep.com/patent/US9290820B2

## CPC

- C12Q 1/701: {Specific hybridization probes}
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 2750/14221: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Nucleic acid molecules derived from sequences of novel human parvovirus B19 variant genomes are provided. Also provided are assays and kits comprising the nucleic acid molecules.

## Claims

### Claim 1

A method for detecting a parvovirus B19 nucleic acid in a sample, the method comprising:
a) performing PCR with at least a portion of the sample using a forward primer, the nucleotide sequence of which consists of SEQ ID NO:145, and a reverse primer, the nucleotide sequence of which consists of SEQ ID NO:146; and
b) determining the presence or absence of an amplicon, wherein the presence of the amplicon is indicative of the presence of the parvovirus B19 nucleic acid in the sample, wherein determining comprises annealing an oligonucleotide to the amplicon.

### Claim 2 (depends on 1)

The method of claim 1, wherein the oligonucleotide comprises the sequence set forth in:
(SEQ ID NO:147) or the sequence fully complementary thereto.

### Claim 3 (depends on 1)

The method of claim 1, wherein the presence of the amplicon indicates the presence of a parvovirus B19 genotype 1 or 3 if present in the sample.