Engineered microorganisms having resistance to ionic liquids
AssigneeLawrence Livermore National Security, LLC
InventorsThomas Lawrence Ruegg, Michael P. Thelen
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Abstract
The present invention provides for a method of genetically modifying microorganisms to enhance resistance to ionic liquids, host cells genetically modified in accordance with the methods, and methods of using the host cells in a reaction comprising biomass that has been pretreated with ionic liquids.
Claims11
A method of increasing the yield from a reaction in which soluble sugars are a source of carbon, the method comprising incubating a microorganism with biomass pretreated with an ionic liquid, wherein the biomass pretreated with an ionic liquid provides a source of soluble sugars as a carbon source, wherein the microorganism comprises a heterologous gene encoding a Major Facilitator Superfamily 1 (MFS 1) polypeptide operably linked to a promoter, wherein said MFS 1 polypeptide has an amino acid sequence with at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, and wherein said MFS 1 polypeptide facilitates resistance of the microorganism to said ionic liquid as compared to a microorganism without said heterologous gene.
Dependencies are inferred from the claim text.