Methods and systems for image data processing
AssigneeLuminex Corporation
InventorWayne D. Roth
Life of this patent
Estimating the term…
Abstract
Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.
Claims20
A method, comprising:
- a computing system receiving a first normalization set of one or more images of an imaging region containing first particles having a first known concentration of fluorescence material associated therewith;
- the computing system receiving a second normalization set of one or more images of the imaging region containing second particles having a second known concentration of fluorescence material associated therewith;
- the computing system determining, based on the received first and second normalization sets, normalization parameters for selected portions of the imaging region;
- the computing system receiving a data set of one or more images of the imaging region containing particles having an unknown concentration of fluorescence material associated therewith;
- and
- the computing system normalizing the data set based on the normalization parameters.
An apparatus, comprising:
- at least one processor;
- and
- an imaging subsystem configured to accept a plurality of particles in an imaging region of the apparatus, supply light to the imaging region, and capture images of the plurality of particles;
- wherein the at least one processor is configured to:
- receive, from the imaging subsystem, a first normalization set of one or more images of particles having a first known concentration of fluorescence material associated therewith;
- receive, from the imaging subsystem, a second normalization set of one or more images of particles having a second known concentration of fluorescence material associated therewith;
- determine, based on the received first and second normalization sets, normalization parameters for selected portions of the imaging region;
- receive, from the imaging subsystem, a data set of one or more images of particles having an unknown concentration of fluorescence material associated therewith;
- and
- normalize the data set based on the normalization parameters.
A non-transitory, computer-readable medium having instructions coded thereon that, when executed by a computing system, cause the computing system to carry out operations comprising:
- receiving a normalization set of one or more images of an imaging region containing particles having a known concentration of fluorescence material associated therewith;
- determining, based on the received normalization set of one or more images, normalization parameters for selected portions of the imaging region;
- storing the normalization parameters as a matrix having entries corresponding to the selected portions of the imaging region;
- receiving a data set of one or more images of the imaging region containing particles having an unknown concentration of fluorescence material associated therewith;
- and
- normalizing the data set based on the normalization parameters.
Dependencies are inferred from the claim text.