Method for imaging high density biochemical arrays with sub-pixel alignment
AssigneeComplete Genomics, Inc.
InventorsBryan P. Staker, Craig E. Uhrich
Life of this patent
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Abstract
A method and associated system for imaging high density biochemical arrays comprises one or more imaging channels that share a common objective lens and a corresponding one or more time delay integration-type imaging cameras with optical alignment mechanisms that permit independent inter-channel and intra-channel adjustment of each of four degrees: X, Y, rotation and scale. The imaging channels are configured to independently examine different spectra of the image of the biochemical arrays.
Claims8
A method for aligning an imaging system having a microscope objective and a multichannel imager, the method comprising:
- (a) collecting light emitted from a sample on an x-y positioning stage;
- (b) obtaining an image of the sample along a rotational axis of symmetry of the microscope objective;
- (c) calculating a first angle between the multichannel imager and the sample around the rotational axis of symmetry;
- (d) calculating a second angle between the sample and the x-y positioning stage around the axis of rotational symmetry;
- (e) rotating the sample by the second angle to align the sample with the x-y positioning stage;
- and
- (f) rotating the multichannel imager by the sum of the first angle and the second angle to align the camera with the x-y positioning stage.
Dependencies are inferred from the claim text.