US 9,285,376 B2 · Measuring range extension of chromatographic rapid tests

Abstract

A method for the quantitative determination of an analyte in a sample is provided comprising: (a) providing an analyte-specific substance which is able to undergo a reaction which generates a detectable signal when it is contacted with an analyte; (b) providing at least two calibration graphs which have been generated by reacting in each case the same analyte-specific substance with different amounts of in each case the same test analyte for in each case a predetermined reaction time; (c) contacting the analyte-specific substance with a sample which contains the analyte to be detected; (d) measuring the signal at a first predetermined reaction time for which a first calibration graph according to (b) is provided; (c) checking whether the signal measured according to (d) enables a quantitative determination of the analyte with a desired accuracy; (f) (i) quantitatively determining the analyte on the basis of the signal measured according to (d) if the desired accuracy is reached, or (ii) measuring the signal at a second predetermined reaction time for which a second calibration graph according to (b) is provided; (g) checking whether the signal measured according to (f(ii)) enables a quantitative determination of the analyte with a desired accuracy; and (h) (i) quantitatively determining the analyte on the basis of the signal measured according to f(ii) if the desired accuracy is reached, or (ii) continuing the determination at at least one further predetermined reaction time (corresponding to (f)(ii), (g), (h)(i)).

Claims24

Dependent claims

A method for the quantitative determination of an analyte in a sample comprising:

(a) providing a test strip comprising an analyte-specific substance which is able to undergo a reaction which generates a detectable signal when it is contacted with an analyte,
(b) providing at least two calibration graphs which have been generated by reacting in each case the same analyte-specific substance with different amounts of in each case the same test analyte for in each case a predetermined reaction time,
(c) contacting the analyte-specific substance with a sample which contains the analyte to be detected,
(d) measuring a first signal at a first predetermined reaction time for which a first calibration graph according to (b) is provided, wherein the reaction between the analyte to be determined and the analyte specific substance is not in a saturation range or stationary state at the first predetermined reaction time,
(e) measuring a second signal at a second predetermined reaction time for which a second calibration graph according to (b) is provided, wherein the reaction between the analyte to be determined and the analyte specific substance is in a saturation range or stationary state at the second predetermined reaction time,
(f) comparing the signals measured according to steps (d) and (e) to an empirical concentration limit to determine which of the signals enables a sufficient accuracy for the quantitative determination of the analyte to be determined, and
(g) quantitatively determining the analyte to be determined on the basis of the more accurate of the first signal and the second signal.

4 more elements

Dependencies are inferred from the claim text.

Measuring range extension of chromatographic rapid tests

Life of this patent

Measuring range extension of chromatographic rapid tests

Life of this patent

Abstract

Claims24