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US 9,285,363 B2Granted
Method of protein nanostructure fabrication
AssigneeImiplex LLC
InventorsF. Raymond Salemme, Patricia C. Weber
Life of this patent
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Abstract
A method of assembling a protein nanostructure on a surface including selectively patterning a surface with a fixation site, bringing a protein node into contact with the surface, and allowing the protein node to bond with the fixation site, so that the position and/or orientation of the protein node is constrained, and compositions.
Claims13
A protein nanostructure comprising,
- a protein node;
- a selectively patterned surface comprising at least one fixation site, the protein node bound with the at least one fixation site
- a second protein node,
- wherein the protein node comprises an interaction domain and the second protein node comprises a second interaction domain and
- wherein the interaction domain of the protein node interacts with the second interaction domain of the second protein node;
- a streptavidin bound to an adaptor protein comprising a binding domain;
- and
- an antibody protein,
- wherein the antibody protein is bound to the binding domain and
- wherein the streptavidin is bound to the protein node.
7 more elements
A method of assembling a protein nanostructure, comprising:
- providing a protein node;
- providing a surface;
- selectively patterning the surface with a fixation site;
- bringing the protein node into contact with the surface;
- and
- allowing the protein node to bond with the fixation site, so that a position and/or orientation of the protein node is constrained
- providing a second protein node;
- wherein the protein node comprises an interaction domain and the second protein node comprises a second interaction domain;
- allowing the interaction domain of the protein node to interact with the second interaction domain of the second protein node;
- providing a streptavidin;
- providing an adaptor protein comprising a binding domain;
- binding the streptavidin to the adaptor protein;
- providing an antibody protein;
- binding the antibody protein to the binding domain;
- and
- binding the streptavidin to the protein node.
13 more elements
Dependencies are inferred from the claim text.