Vitro method for high throughput screening of genotoxic agents in eukaryotic cells
AssigneeAnthem Biosciences Pvt Ltd.
InventorsAyyappan Nair, Madhuri Subbiah, Gunja Gupta, Lakshmi Rajakrishna, Pradeep Savanoor, Subbulakshmi Karthikeyan, Salini Krishnan Unni, Ganesh Sambasivam
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Abstract
The present invention relates to a novel method for high throughput detection of wide range of genotoxins in eukaryotic cells wherein the eukaryotic cell based tool combines the ability to detect a broad spectrum of genotoxic signaling events and a simple and reproducible assay technique. The present invention further comprises expression cassettes, vectors, and eukaryotic cell lines for the same.
Claims11
A eukaryotic cell comprising a first expression cassette which includes a DNA sequence encoding Renilla Luciferase (RLuc) and derivatives thereof as a reporter protein, operatively linked to human p21 gene promoter;
- a second expression cassette which includes a DNA sequence encoding humanized Firefly Luciferase (hFLuc) and derivatives thereof as a reporter protein, operatively linked to hamster GADD153 gene promoter;
- and a third expression cassette which includes a DNA sequence encoding beta galactosidase (Bgal) and derivatives thereof as reporter protein, operatively linked to p53 response element and polyoma virus UTR as promoter.
Dependencies are inferred from the claim text.