Bubble reduction device, chromatography device, bubble reduction method, and bubble reduction program

A bubble reduction method comprising:

• a liquid accommodation step to accommodate a liquid into a liquid accommodation portion that is to elute an analysis component from a specimen adsorbed to an adsorption portion,
• a liquid supply step, which by pushing up or down a rod of the liquid supply apparatus, sucks or discharges the liquid from the liquid accommodation portion through an aperture portion of a tube portion, the aperture portion being oriented upward;
• a step to supply liquid discharged from the liquid supply apparatus to the adsorption portion, through which a first channel that connects the liquid supply apparatus with the liquid accommodation portion and a second channel that is connected to the first channel via a second switching valve, the second channel supplying the liquid discharged from the liquid supply apparatus to the adsorption portion;
• an air layer formation step of forming an air layer in the tube portion and taking bubbles in the liquid into the air layer;
• an evacuation step of evacuating the air layer in the tube portion through an evacuation portion that is connected to the first channel via a first switching valve and evacuates the air layer through the first channel;
• a step to release the atmosphere through an atmosphere release valve that is provided at at least one of the tube portion, the first channel or the second channel, and that introduces the air layer into the tube portion;
• and
• an analyzing step to analyze using an analyzer that analyzes the analysis component in the liquid passed through the adsorption portion.