Loading patent…
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorOtto Phanstiel, IV
Estimating the term…
Cyano-substituted anthracene containing polyamines were synthesized and shown to be efficient polyamine transporter ligands. Moreover, these compounds (3 and 4) had improved fluorescence properties over previously known anthryl-polyamine conjugates, which facilitated their intracellular trafficking by confocal microscopy and fluorescence methods. These cytotoxic fluorescent agents may find use as molecular probes which traffic into cells via the polyamine transport system and may also be viable anticancer drugs which are readily quantified in human tissues due to their excellent fluorescence properties: (excitation: λ 405 nm) and emission (420 nm) occurs in the visible light range. The ability to excite and emit in the visible range provides an advantage to these probes as these wavelengths are considered non-toxic to human cells (versus ultraviolet mediated excitations, λ<400 nm) and visible light lasers are less costly to purchase and operate than UV laser sources.
A method of screening for cells or cell lines that are susceptible to a polyamine transport system targeting strategy, the method comprising:
A method for screening cancer cells for polyamine transport activity comprising:
A method of screening for therapeutic drug candidates that modulate uptake of polyamines in cells, said method comprising
Dependencies are inferred from the claim text.