Device, system, and method for depositing processed immiscible-fluid-discrete-volumes

A method comprising:

• diluting a sample into sample portions wherein at least one sample portion contains a single fragment of target analyte;
• forming in a conduit a plurality of spaced apart aqueous sample slugs comprising at least one target analyte comprising at least one respective linkage group, wherein the forming includes:
• flowing the sample portions and the spacing fluid from an aqueous sample injection unit and a spacing fluid injection unit, respectively, and
• injecting volumes of the sample portions and the spacing fluid respectively to form the aqueous sample slugs in the conduit;
• amplifying a target analyte located in at least one aqueous sample slug;
• dispensing the aqueous sample slugs one-at-a-time from the conduit onto a substrate to form a pattern of aqueous samples on the substrate, the substrate comprising an electrically conductive surface adapted to bind the at least one respective linkage group to form an attached analyte and an immersion fluid configured to have the dispensed aqueous sample slugs immersed therein, and the dispensing onto the substrate comprising dispensing the aqueous sample slugs directly onto the electrically conductive surface of said substrate;
• reacting the at least one target analyte with a reagent mixture to form at least one target analyte product;
• imaging the electrically conductive surface to differentiate a target analyte product from non-target analyte products;
• positioning a collection device comprising a capillary of a capillary electrophoretic analyzer, adjacent the at least one target analyte product;
• injecting at least a portion of the at least one target analyte product into the collection device wherein the injecting comprises applying a potential to the electrically conductive surface and the capillary;
• and
• performing nucleic acid sequencing on the at least one target analyte product with the capillary electrophoretic analyzer.

A method comprising:

• diluting a sample into sample portions wherein at least one sample portion contains a single fragment of target analyte;
• flowing the sample portions and the spacing fluid from an aqueous sample injection unit and a spacing fluid injection unit, respectively, and
• injecting volumes of the sample portions and the spacing fluid respectively to form aqueous sample slugs in the conduit;
• amplifying target analyte in a plurality of aqueous sample slugs in a conduit to form amplicons, each aqueous slug separated from an adjacent aqueous slug by at least one oil slug;
• depositing the amplicons from the conduit onto the substrate, the substrate comprising an electrically conductive surface and an immersion fluid configured to have the deposited amplicons immersed therein, and the depositing comprising depositing the amplicons directly onto the electrically conductive substrate;
• attaching the amplicons to the electrically conductive surface;
• contacting the substrate with a sequencing reaction mixture to form at least one dye-labeled spot;
• imaging the electrically conductive surface to differentiate a target analyte product from non-target analyte products;
• positioning a capillary of a capillary electrophoretic analyzer over the at least one dye-labeled spot;
• electrically contacting the dye-labeled spot with the capillary;
• injecting one or more components from the dye-labeled spot into the capillary wherein the injecting comprises applying a potential to the electrically conductive surface and the capillary;
• and
• performing nucleic acid sequencing on the at least one target analyte product with the capillary electrophoretic analyzer.

A method comprising:

• diluting a sample into sample portions wherein at least one sample portion contains a single fragment of target analyte;
• flowing the sample portions and the spacing fluid from an aqueous sample injection unit and a spacing fluid injection unit, respectively, and
• injecting volumes of the sample portions and the spacing fluid respectively to form aqueous sample slugs in a conduit, each aqueous slug separated from an adjacent aqueous slug by at least one non-aqueous slug;
• moving the conduit comprising the target analyte over a substrate and depositing the target analyte from the conduit onto the substrate, the substrate comprising an electrically conductive surface and an immersion fluid configured to have the dispensed aqueous sample slugs immersed therein, and the depositing comprising depositing the aqueous sample slugs directly onto the electrically conductive substrate;
• attaching the target analyte to the electrically conductive surface;
• contacting the electrically conductive surface with a reaction mixture;
• imaging the electrically conductive surface to differentiate a target analyte product from non-target analyte products;
• positioning a second conduit of a capillary electrophoretic analyzer over the target analyte product;
• electrically contacting the target analyte product with the second conduit;
• injecting the target analyte product into the second conduit wherein the injecting comprises applying a potential to the electrically conductive surface and the second conduit;
• and
• performing nucleic acid sequencing on the at least one target analyte product with the capillary electrophoretic analyzer.