US 9,284,604 B2Granted

Methods of identifying a cellular nascent RNA transcript

AssigneeThe Regents of the University of California

InventorsJonathan Weissman, Lee Stirling Churchman

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Methods and compositions for identifying a cellular nascent RNA transcript are provided.

Dependent claims

A method of identifying a cellular nascent RNA transcript, comprising:

(a) arresting transcription in a cell;
(b) purifying a native cellular nascent RNA transcript;
and
(c) sequencing said native cellular nascent RNA transcript thereby identifying the cellular nascent RNA transcript;
wherein said native cellular nascent RNA transcript is purified as part of an RNA polymerase complex, wherein said cellular nascent RNA transcript does not comprise a labeled nucleotide and wherein said cellular nascent RNA transcript is not crosslinked to an RNA polymerase complex.

2 more elements

Dependencies are inferred from the claim text.

C12Q 1/6869
Involving nucleic acids: methods for sequencing
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6809
Involving nucleic acids: methods for determination or identification of nucleic acids involving differential detection
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

From the US filing history. Foreign family members aren’t listed.

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