Methods for determining enzymatic activity comprising heating and agitation of closed volumes

A method for determining enzymatic activity, the method comprising:

• heating to at least a predetermined reaction temperature a conductive structure supporting sealed reaction vessels containing substrate;
• substantially simultaneously injecting at least one enzyme into the sealed reaction vessels;
• while heating the conductive structure, agitating the conductive structure supporting the sealed reaction vessels with the substrate and the at least one enzyme in a plane of motion parallel to a length of the sealed reaction vessels;
• and
• determining activity of the at least one enzyme.

A method for determining enzymatic activity, the method comprising:

• heating a structure comprising vessels to at least a predetermined reaction temperature;
• replacing the vessels with other vessels, at least some vessels of the other vessels comprising a substrate sample in a fluid and sealed with a penetrable lid;
• at at least the predetermined reaction temperature and without opening the other vessels, injecting, through the penetrable lid and into the fluid, at least one enzyme;
• while heating the structure comprising the other vessels, moving the other vessels through a horizontal plane to intermix the substrate sample and the at least one enzyme;
• and
• determining activity of the at least one enzyme.