US 9,284,595 B2Granted

Real-time assay for the detection of botulinum toxin

AssigneeSRC, Inc.

InventorsKenton Arthur Doctor, Stacey Ann Massulik, Frances Louise Stites, Timothy Francis Moshier, Jeffrey Harold Mills, Lisa Helen Chamberlin, Deborah Lynne Plochocki, Olivia Jennifer Barrett, Huda Sirageldin Suliman

Life of this patent

Estimating the term…

patentgrep

Loading patent…

Abstract

A real-time portable and rapid detection assay to identify the presence of biologically active toxins such as botulinum toxins. The proteolytic activity of BoNT/A is measured using a peptide cleavage assay, where a fluorescent substrate is cleaved by BoNT/A, resulting in increased fluorescence. This fluorescence can be monitored in real-time using a fluorescence detection instrument, such as a real-time PCR system that has been modified to implement a detection algorithm specific to the identification of the target toxin.

Claims7

Dependent claims

A method for detecting the presence of biologically active botulinum toxin, comprising the steps of:

providing a peptide substrate comprising SEQ. ID NO. 1 labeled with a fluorophore and a quencher and having a cleavage site responsive to biologically active botulinum toxin positioned between said fluorophone and said quencher;
adding a test sample to said peptide substrate so that any biologically active botulinum toxin contained in said test sample will cleave said peptide substrate, thereby allowing said peptide substrate to fluoresce;
incubating said peptide substrate;
detecting the amount of fluorescence emitted from said sample and outputting a corresponding fluorescence reading;
determining whether any biologically active botulinum toxin is present in said sample based on said fluorescence reading.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/568,220Filed 12 Dec 2014 · division
Priority: 3 Dec 2012
Published: US 2015/0159194 A111 Jun 2015
Granted: 15 Mar 2016
Term ends: Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12Q 1/37
Involving hydrolase: involving peptidase or proteinase
C07K 7/00
Peptides having 5 to 20 amino acids in a fully defined sequence
G01N 33/56911
For microorganisms, e.g. protozoa, bacteria, viruses: bacteria
G01N 2333/33
From bacteria: from Clostridium

Family

Application61/732,436Us provisional application · filed 3 Dec 2012
Application13/839,827Parent of this division · filed 15 Mar 2013
US 2015/0159194 A1Pre-grant publication · published 11 Jun 2015
US 8,962,340 B2Parent of this division
US 2014/0154812 A1Published application
US 9,284,595 B2This patent

From the US filing history. Foreign family members aren’t listed.