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US 9,284,593 B2Granted
Live bioload detection using microparticles
Assignee3M Innovative Properties Company
InventorsRaj Rajagopal, Kurt J. Halverson, Manjiri T. Kshirsagar, James E. Aysta
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Abstract
The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.
Claims8
A unitary sample preparation and detection device, comprising:
- a housing comprising first and second receptacles with a passageway there between;
- wherein the first receptacle comprises an opening configured to receive a sample and a cell concentration agent disposed therein;
- wherein the second receptacle includes a detection reagent disposed therein;
- a release element comprising a cell extractant, the release element disposed in the housing;
- means for isolating the first receptacle from the second receptacle and transferring the cell concentration agent from the first receptacle to the second receptacle;
- wherein the means for isolating the first and second receptacles of the housing includes a valve;
- wherein the release element comprises an encapsulating material that acts as a diffusion barrier to prevent instantaneous release of an effective amount of the cell extractant from the release element into an aqueous liquid when the release element contacts the aqueous liquid;
- and
- wherein the valve comprises a dead-end valve.
6 more elements
Dependencies are inferred from the claim text.