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US 9,284,584 B2Granted
Method for producing an L-amino acid using a bacterium of the family Enterobacteriaceae having enhanced expression of the flagella formation and motility cascade genes
AssigneeAjinomoto Co., Inc.
InventorsIrina Borisovna Altman, Tatyana Abramovna Yampolskaya, Leonid Romanovich Ptitsyn
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Abstract
The present invention provides a method for producing L-amino acid using a bacterium belonging to the family Enterobacteriaceae, particularly a motile bacterium belonging to the genus Escherichia, Enterobacter or Pantoea, wherein the bacterium has been modified so that expression of at least one gene of the flagella formation and motility cascade is enhanced.
Claims10
A method for producing an L-amino acid comprising culturing an Escherichia coli bacterium in a medium, and collecting the L-amino acid from the medium,
- wherein the bacterium is able to produce an L-amino acid and has been modified so that expression of either the flhD gene or flhC gene, or both, is/are enhanced by a method selected from the group consisting of:
- a) increasing the copy number of the gene(s), b) modifying an expression control region of the gene(s), and c) combinations thereof;
- wherein the flhD gene encodes a protein selected from the group consisting of:
- (A) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 2;
- (B) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 2, but wherein one to five amino acid residues are substituted, deleted, inserted, added or inverted, and said protein has DNA-binding transcriptional dual regulator activity according to the amino acid sequence of SEQ ID NO:
- 2, and
- (C) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 2, and said protein has DNA-binding transcriptional dual regulator activity according to the amino acid sequence of SEQ ID NO:
- 2, and
- (D) combinations thereof;
- wherein the flhC gene encodes a protein selected from the group consisting of:
- (i) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 4,
- (ii) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 4, but wherein one to five amino acid residues are substituted, deleted, inserted, added or inverted, and said protein has DNA-binding transcriptional dual regulator activity according to the amino acid sequence of SEQ ID NO:
- 4,
- (iii) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 4, and said protein has DNA-binding transcriptional dual regulator activity according to the amino acid sequence of SEQ ID NO:
- 4, and
- (iv) combinations thereof.
19 more elements
Dependencies are inferred from the claim text.