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AssigneeUnist Academy-Industry Research Corporation
InventorsSung Kuk Lee, Goo Hee Kim, Seong Hun Jeong, Suk Min Kim, Bae Young Choi
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The present invention relates to a method for preparing a mutant E. coli strain, capable of simultaneously using glucose and xylose, by genetic engineering and evolutionary adaptation; the mutant E. coli prepared using the same; and a method for producing biofuels, biologically active ingredients, medicinal materials or base chemicals for the chemical industry using the mutant E. coli. Being capable of simultaneously using glucose and xylose, in contrast to wild-type E. coli, the mutant E. coli can be effectively applied to the enzymatic saccharification process of producing biofuels from a biomass.
A method for preparing a mutant E. coli capable of simultaneously utilizing glucose and xylose from a wild-type E. coli, comprising:
A mutant E. coli, capable of simultaneously utilizing glucose and xylose, prepared by replacing inducible promoters of araBAD operon, araFGH operon, araE gene, xylAB operon, and xylFGH operon of wild-type E. coli with respective constitutive promoters, and growing the promoter-replaced E. coli in a xylose minimal medium or an arabinose and xylose minimal medium for 10 days or longer.
A method for producing a biofuel, a biologically active ingredient, a medicinal material, or a chemical substance for the chemical industry from a biomass by using a mutant E. coli capable of simultaneously utilizing glucose and xylose, said mutant E. coli being prepared by replacing inducible promoters of araBAD operon, araFGH operon, araE gene, xylAB operon, and xylFGH operon of a wild-type E. coli with respective constitutive promoters, and growing the promoter-replaced E. coli in a xylose minimal medium or an arabinose and xylose minimal medium for 10 days or longer.
Dependencies are inferred from the claim text.