Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol

A non-naturally occurring microbial organism comprising:

• (1) a Wood-Ljungdahl pathway, wherein said microbial organism comprises at least one exogenous nucleic acid encoding a Wood-Ljungdahl pathway enzyme expressed in a sufficient amount to enhance carbon flux through acetyl-CoA, wherein said Wood-Ljungdahl pathway enzyme is selected from the group consisting of:
• a) a formate dehydrogenase that catalyzes the incorporation of CO2 into formate;
• b) a formyltetrahydrofolate synthetase that ligates formate to tetrahydrofolate to form 10-formyltetrahydrofolate,
• c) a methenyltetrahydrofolate cyclohydrolase that converts 10-formyltetrahydrofolate to methyltetrahydrofolate,
• d) a methylenetetrahydrofolate dehydrogenase that converts methyltetrahydrofolate to methenyltetrahydrofolate,
• e) a methylenetetrahydrofolate reductase that converts metheneyltetrahydrofolate to 5-methyltetrahydrofolate;
• f) a methyltetrahydrofolate:corrinoid protein methyltransferase that catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to corrinoid iron sulfur protein,
• g) a corrinoid iron-sulfur protein,
• h) a nickel-protein assembly protein,
• i) a ferredoxin,
• j) a acetyl-CoA synthase that catalyzes the condensation of the methylated corrinoid iron sulfur protein, carbon monoxide and coenzyme A, yielding acetyl-CoA,
• k) a carbon monoxide dehydrogenase that converts CO and water to CO2 while passing the electrons to a reduced acceptor,
• l) a pyruvate ferredoxin oxidoreductase that converts the pyruvate to acetyl-CoA or pyruvate dehydrogenase that converts the pyruvate to acetyl-CoA, and
• m) a pyruvate formate lyase that converts the pyruvate and CoA to acetyl-CoA;
• and
• (2) a 1,3-butanediol pathway that converts acetyl-CoA to 1,3-butanediol, wherein the 1,3-butanediol pathway comprises at least three enzymes selected from the group consisting of:
• a) an acetoacetyl-CoA thiolase that converts acetyl-CoA to acetoacetyl-CoA,
• b) an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming) that converts acetoacetyl-CoA to 4-hydroxy,2-butanone,
• c) a 3-oxobutyraldehyde reductase (aldehyde reducing) that converts 3-oxobutyraldehyde into 4-hydroxybutanone,
• d) a 4-hydroxy,2-butanone reductase that converts 4-hydroxy,2-butanone to 1,3-butanediol,
• e) an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming) that converts acetoacetyl-CoA to 3-oxobutyraldehyde,
• f) a 3-oxobutyraldehyde reductase (ketone reducing) that converts 3-oxobutyraldehyde to 3-hydroxybutyraldehyde,
• g) a 3-hydroxybutyraldehyde reductase that converts 3-hydroxybutyraldehyde to 1,3-BDO,
• h) an acetoacetyl-CoA reductase (ketone reducing) that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA,
• i) a 3-hydroxybutyryl-CoA reductase (aldehyde forming) that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde,
• j) a 3-hydroxybutyryl-CoA reductase (alcohol forming) that converts 3-hydroxybutyryl-CoA to 1,3-butanediol,
• k) an acetoacetyl-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA synthetase, or a phosphotransacetoacetylase/acetoacetate kinase that converts acetoacetyl-CoA to acetoacetate,
• l) an acetoacetate reductase that converts acetoacetate to 3-oxobutyraldehyde,
• m) a 3-hydroxybutyryl-CoA transferase, a 3-hydroxybutyryl-CoA hydrolase, or a 3-hydroxybutyryl-CoA synthetase that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate,
• n) a 3-hydroxybutyrate reductase that converts 3-hydroxybutyrate to 3-hydroxybutyraldehyde;
• and
• o) a 3-hydroxybutyrate dehydrogenase that converts acetoacetate to 3-hydroxybutyrate.