Rag-based selection methods for improving aphid resistance in soybeans

A method of improving resistance to one or more soybean aphid biotypes in a soybean plant, line, or strain, the improved resistance comprising one or more of improved antibiosis resistance and improved antixenosis resistance;

• the method comprising:
• a) identifying in a first soybean plant or soybean germplasm, or a soybean progeny thereof, a favorable allele of at least one marker of comprising S07165-1-Q3;
• b) selecting said first soybean plant, soybean germplasm, or soybean progeny from step a);
• c) identifying in a second soybean plant or soybean germplasm, or a soybean progeny thereof, a favorable allele of at least one marker of at least one Rag gene, wherein said Rag gene is selected from the group consisting of Rag1, Rag2, and Rag3;
• wherein said Rag gene of said second soybean plant, soybean germplasm, or soybean progeny is different than the Rag gene of said first soybean plant, soybean germplasm, or soybean progeny;
• d) selecting said second soybean plant, soybean germplasm, or soybean progeny from step c);
• and
• e) crossing said first soybean plant, soybean germplasm, or soybean progeny with said second soybean plant, soybean germplasm, or soybean progeny, wherein said crossing produces a soybean progeny comprising (i) said favorable allele of the at least one marker from said first soybean plant, soybean germplasm, or soybean progeny;
• and (ii) said favorable allele of the at least one marker from said second soybean plant, soybean germplasm, or soybean progeny.