Use of a seed specific promoter to drive ODP1 expression in cruciferous oilseed plants to increase oil content while maintaining normal germination
AssigneeE. I. Du Pont de Nemours and Company
InventorsHoward Glenn Damude, John D. Everard, Knut Meyer, Kevin G. Ripp, Kevin L. Stecca
Life of this patent
Estimating the term…
Abstract
A recombinant DNA construct comprising a polynucleotide encoding an ODP1 polypeptide operably linked to a sucrose synthase 2 promoter where this construct can be used to increase oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination is disclosed. A method for increasing oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination using this construct is also disclosed.
Claims23
A recombinant DNA construct comprising a polynucleotide encoding an ODP1 polypeptide operably linked to a sucrose synthase 2 promoter (SUS2) wherein the SUS2 promoter comprises a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:43, wherein said nucleotide sequence has seed specific promoter activity and wherein the amino acid sequence of said ODP1 polypeptide has at least 90% sequence identity to SEQ ID NO:39 and comprises two APETALA2 (AP2) domains and wherein expression of said ODP1 polypeptide increases oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination.
A method for increasing oil content in seeds of a transgenic cruciferous oilseed plant while maintaining normal germination, said method comprising:
- (a) transforming a cruciferous oilseed plant cell with a recombinant DNA construct comprising a polynucleotide encoding an ODP1 polypeptide, wherein the amino acid sequence of said ODP1 polypeptide has at least 90% sequence identity to SEQ ID NO:39 and comprises two APETALA2 (AP2) domains, said polynucleotide being operably linked to a promoter having a nucleotide sequence at least 95% identical to SEQ ID NO:
- 43, wherein said nucleotide sequence has seed specific promoter activity;
- (b) regenerating a transgenic cruciferous oilseed plant from the transformed cell of step (a), wherein said plant comprises the recombinant DNA construct;
- (c) obtaining a transgenic progeny plant derived from the transgenic cruciferous oilseed plant of step (b), wherein the transgenic progeny plant comprises in its genome the recombinant DNA construct;
- (d) assaying the transgenic progeny plant obtained from step (c) for oil level and germination;
- and
- (e) selecting those transgenic progeny plants having seeds comprising said recombinant DNA construct and having an increased level of oil and normal germination when compared to seeds obtained from a control cruciferous oilseed plant, wherein said control plant does not comprise the recombinant DNA construct.
Dependencies are inferred from the claim text.