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US 9,284,569 B2Granted
Autoluminescent phytosensor plants and uses thereof
AssigneeBioglow Inc.
InventorAlexander Krichevsky
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Abstract
Real-time monitoring of plant or environmental conditions is solved by Autoluminescent Phytosensor Plants (ALPS) disclosed herein that emit light in response to a specified stimulus or condition, which light emission is detected or measured by a sensor.
Claims3
A transplastomic plant cell containing a LUX operon comprising LUX genes integrated within a plastidal genome therein,
- wherein any or all of said LUX genes are separated by an intercistronic expression element (IEE) operably linked thereto,
- wherein expression of said LUX genes is enhanced by a heterologous translational leader sequence operably linked to one or more of said LUX genes,
- wherein said heterologous translational leader sequence is a T7g10 leader sequence, and
- wherein said transplastomic plant cell is selected from the group consisting of a species of petunia and a species of Nicotiana.
1 more element
A method of producing an autoluminescent plant comprising:
- introducing into a plant cell plastid an expression cassette containing a LUX operon comprising LUX genes,
- wherein any or all of said LUX genes are separated by an intercistronic expression element (IEE) operably linked thereto,
- wherein expression of said LUX genes is enhanced by a heterologous translational leader sequence operably linked to one or more of said LUX genes,
- wherein said heterologous translational leader sequence is a T7g10 leader sequence,
- wherein said plant cell plastid is regenerated into a mature transplastomic plant, and
- wherein said transplastomic plant is selected from the group consisting of a species of petunia and a species of Nicotiana.
3 more elements
Dependencies are inferred from the claim text.