Method for gene introduction into hordeum plant using agrobacterium, and method for production of transformed plant of hordeum plant

A method of producing a transformed Hordeum plant, the method comprising the steps of:

• (i) coculture step by inoculating a barley immature embryonic tissue with Agrobacterium and coculturing the tissue in the presence of the Agrobacterium in a coculture medium satisfying at least one of the following conditions a) to c):
• a) containing an antiauxin;
• b) containing a cytokinin;
• and
• c) containing an antiauxin, and containing a phenoxy auxin in an amount of less than 2 μM and/or a benzoic auxin in an amount of less than 5 μM, or not containing any phenoxy auxin and/or benzoic auxin;
• (ii) a step of subjecting the tissue to a centrifugation treatment and/or a pressurization treatment before the inoculation with Agrobacterium, during the coculture step, and/or after the coculture step;
• (iii) resting step by culturing the tissue in a resting medium;
• and
• (iv) regeneration step by regenerating the tissue in a regeneration medium.