Recombinant microorganisms comprising stereospecific diol dehydratase enzyme and methods related thereto
AssigneeLanzatech New Zealand Limited
InventorsAlexander Paul Mueller, Michael Koepke
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Abstract
A stereospecific enzyme in C. autoethanogenum permits the conversion of racemic propanediol to acetone and/or propionaldehyde. Entantiomeric starting materials lead to different products. If desired, the products may be reduced to form alcohols. The reaction can be performed in various host cells, so that various materials may be used as carbon and/or energy sources.
Claims19
A recombinant microorganism comprising heterologous nucleic acids encoding (a) the stereospecific diol dehydratase of SEQ ID NO:
- 1, and (b) the activase of SEQ ID NO:
- 2.
A recombinant Clostridum autoethanogenum microorganism comprising nucleic acids encoding (a) the stereospecific diol dehydratase of SEQ ID NO:
- 1, and (b) the activase of SEQ ID NO:
- 2, wherein the microorganism has been genetically modified to increase the expression of the nucleic acids encoding the stereospecific diol dehydratase and activase compared to the unmodified wild-type Clostridum autoethanogenum.
A recombinant Clostridum autoethanogenum microorganism comprising nucleic acids encoding (a) the stereospecific diol dehydratase of SEQ ID NO:
- 1, and (b) the activase of SEQ ID NO:
- 2, wherein the microorganism has been genetically modified to increase the copy number of the nucleic acids encoding the stereospecific diol dehydratase and activase compared to the unmodified wild-type Clostridum autoethanogenum.
Dependencies are inferred from the claim text.